Journal: Frontiers in Veterinary Science

Article Title: Antiviral activity of nitazoxanide against pseudorabies virus infection in vitro

doi: 10.3389/fvets.2025.1623545

Figure Lengend Snippet: The chemical structure and cytotoxicity of NTZ on PK15 and Vero cell lines. (A) The chemical structure of NTZ. (B,C) PK15 or Vero cells were incubated with NTZ (0–200 μM) for 48 h, the cell viability was assessed using a CCK-8 method (%).

Article Snippet: NTZ ( ) was purchased from MedChemExpress and dissolved in dimethyl sulfoxide (DMSO) to achieve a final concentration of 250 mM.

Techniques: Incubation, CCK-8 Assay

Journal: Frontiers in Veterinary Science

Article Title: Antiviral activity of nitazoxanide against pseudorabies virus infection in vitro

doi: 10.3389/fvets.2025.1623545

Figure Lengend Snippet: Effects of NTZ on different phases of PRV infection in Vero cells. (A) A schematic representation of virus inactivation (I), pre-treatment (II), co-treatment (III), and post-treatment (IV), then cells and supernatants were harvested for western blotting (B) and viral titer determination (C) . (D,E) A schematic representation and results of virus attachment (V), virus entry (VI), and virus release assays (VII).

Article Snippet: NTZ ( ) was purchased from MedChemExpress and dissolved in dimethyl sulfoxide (DMSO) to achieve a final concentration of 250 mM.

Techniques: Infection, Virus, Western Blot

Journal: Frontiers in Veterinary Science

Article Title: Antiviral activity of nitazoxanide against pseudorabies virus infection in vitro

doi: 10.3389/fvets.2025.1623545

Figure Lengend Snippet: Antiviral property of NTZ against the PRV HuN-LD strain in vitro . (A–C,G) Vero cells were pre-treated with varying concentrations of NTZ (12.5, 25.0, and 50.0 μM), and then infected with PRV at a MOI of 0.1. At 24 hpi, both the cells and supernatants were collected for RT-qPCR (A) , TCID 50 (B) , western blotting (C) , and IFA (G) assays to evaluate viral replication. (D–F,G) PK15 cells were pre-treated with varying concentrations of NTZ (12.5, 25.0, and 50.0 μM), and then infected with PRV at a MOI of 0.1. Following a 24-hpi period, both the cells and supernatants were harvested for RT-qPCR (D) , TCID 50 (E) , western blotting (F) , and IFA (G) assays to evaluate viral replication. (H) Vero cells were pre-treated with NTZ (50.0 μM) for 1 h, and then infected with PRV at MOIs of 0.5, 0.1 or 0.02. Supernatants and cells were collected at 24 hpi to quantify viral titers.

Article Snippet: NTZ ( ) was purchased from MedChemExpress and dissolved in dimethyl sulfoxide (DMSO) to achieve a final concentration of 250 mM.

Techniques: In Vitro, Infection, Quantitative RT-PCR, Western Blot

Journal: Frontiers in Veterinary Science

Article Title: Antiviral activity of nitazoxanide against pseudorabies virus infection in vitro

doi: 10.3389/fvets.2025.1623545

Figure Lengend Snippet: Transcriptomic profiling analysis of the potential cellular pathway regulated by NTZ. (A) PCA analysis of all RNA-Seq samples. (B) Differentially expressed genes (DEGs) identified between the mock-infected PK15 cells and PRV-infected PK15 cell, with red and green dots denoting significantly upregulated and downregulated genes, respectively. (C) DEGs between the mock PRV-infected PK15 cells and NTZ-treated PRV-infected PK15 cells. The red and green dots represent the significantly upregulated and downregulated genes, respectively. (D,E) Gene Ontology (D) and Kyoro Encyclopedia of Genes and Genomes (E) enrichment analyses of DEGs between PRV-infected PK15 cells and NTZ-treated PRV-infected PK15 cells. (F) Heat map analysis of the DEGs associated with the oxidative stress pathway, where red and blue colors represent upregulated and downregulated DEGs, respectively. (G) Validation of the mRNA expression levels of selected DEGs identified in the RNA-Seq data though RT-qPCR.

Article Snippet: NTZ ( ) was purchased from MedChemExpress and dissolved in dimethyl sulfoxide (DMSO) to achieve a final concentration of 250 mM.

Techniques: RNA Sequencing, Infection, Biomarker Discovery, Expressing, Quantitative RT-PCR

Journal: iScience

Article Title: Unveiling transcriptional mechanisms of B7-H3 in breast cancer stem cells through proteomic approaches

doi: 10.1016/j.isci.2025.112218

Figure Lengend Snippet:

Article Snippet: Nitazoxanide , Selleckchem , S1627; CAS: 55981-09-4.

Techniques: Recombinant, Transfection, Reporter Assay, cDNA Synthesis, Expressing, Plasmid Preparation, Software

Journal: iScience

Article Title: Unveiling transcriptional mechanisms of B7-H3 in breast cancer stem cells through proteomic approaches

doi: 10.1016/j.isci.2025.112218

Figure Lengend Snippet:

Article Snippet: Nitazoxanide , Selleckchem , S1627; CAS: 55981-09-4.

Techniques: Recombinant, Transfection, Reporter Assay, cDNA Synthesis, Expressing, Plasmid Preparation, Software